Illumina HiSeq 2500
强大高效,适合大规模基因组学
HISEQ SBS V4 PRE-RELEASE SPECIFICATIONS | TRUSEQ SBS V3 | |||||
---|---|---|---|---|---|---|
Read length | Dual Flow Cell | Single Flow Cell | Dual Flow Cell Run Time | Dual Flow Cell | Single Flow Cell | Dual Flow Cell Run Time |
1×36 | 128-144 Gb | 64-72 Gb | 29 hrs | 95-105 Gb | 47-52 Gb | 2 days |
2×50 | 360-400 Gb | 180-200 Gb | 2.5 days | 270-300 Gb | 135-150 Gb | 5.5 days |
2×100 | 720-800 Gb | 360-400 Gb | 5 days | 540-600 Gb | 270-300 Gb | 11 days |
2×125 | 900-1 Tb | 450-500 Gb | 6 days | N/A | N/A | N/A |
Reads Passing Filter | Up to 4 billion single read or 8 billion paired-end reads | Up to 2 billion single read or 4 billion paired-end reads | Up to 3 billion single read or 6 billion paired-end reads | Up to 1.5 billion single read or 3 billion paired-end reads | ||
Quality |
Greater than 85% of bases above Q30 at 2×50 bp Greater than 80% of bases above Q30 at 2×100 bp Greater than 80% of bases above Q30 at 2×125 bp |
Greater than 85% of bases above Q30 at 2×50 bp Greater than 80% of bases above Q30 at 2×100 bp |
*Install specifications based on Illumina PhiX control library at supported cluster densities (between 610-678 K clusters/mm2 passing filter using TruSeq v3 Kits or 950-1050 K clusters/mm2 passing filter using HiSeq v4). Run times high output mode correspond to sequencing only. Performance may vary based on sample quality, cluster density, and other experimental factors.
Read length | Dual Flow Cell | Single Flow Cell | Dual Flow Cell Run Time |
1×36 | 18-22 Gb | 9-11 Gb | 7 hr |
2×50 | 50-60 Gb | 25-30 Gb | 16 hr |
2×100 | 100-120 Gb | 50-60 Gb | 27 hr |
2×150 | 150-180 Gb | 75-90 Gb | 40 hr |
Reads Passing Filter | Up to 600 million single read or 1.2 billion paired-end reads | Up to 300 million single read or 600 million paired-end reads | |
Quality |
Greater than 85% of bases above Q30 at 2×50 bp Greater than 80% of bases above Q30 at 2×100 bp Greater than 75% of bases above Q30 at 2×150 bp |
让应用更轻松,让您专注于研究
从 样品制备到数据分析,我们的重点是让应用更轻松 – 这样您的重点就能放在您的研究上。靶向重测序、基因表达、全基因组测序、表观遗传学,以及更多 – 我们设计了完整的解决方案,以简化您想要开展的任何研究。无论您的研究目标、实验室规模或经费如何,HiSeq 2500系统都为您所有的高通量测序需求提供了一个理想的解决方案。
全基因组测序就在您的实验室
在 您的实验室里就能快速经济地开展基因组测序。选择HiSeq 2500系统的快速运行模式,加速周转时间,在一天内测序整个人类基因组。或者,保持高产量模式,在单次运行中测序多个基因组,假定每个基因组为100 Gb。两种模式都产生了较好的数据质量,带来准确、完整的全基因组测序结果。
靶向重测序
从单个定制panel到整个外显子组,从一整套靶向重测序方案中选择,以支持所有研究设计。经济高效且可扩展的探索、验证和筛查如今触手可及。
全转录组测序
从高通量的基因表达谱分析到深度的转录分析,HiSeq提供了前所未有的RNA分析方案。测定单个转录本和亚型的丰度,发现新的基因,并鉴定非编码调控RNA。
De Novo 测序
有了高产量、长reads和双端测序的独特组合,HiSeq成为任何大小基因组de novo测序的强大工具。通过测序整个微生物群落,开展真正的宏基因组分析,以发现重要的分类和功能信息。
表观遗传调控
在HiSeq单次运行中,通过评估多种形式的表观遗传调控,了解更加完整的生物学故事。利用ChIP-Seq研究DNA与蛋白的互作和基因调控。以单碱基分辨率定量DNA甲基化。